Restek - HPLC Columns and Accessories
LC-MS HPLC Column Selection

try New Pinnacle DB 1.9um x 2.1mmID PDF
 Flowopt 0.4ml/min 240K plates/metre

C18, UPFP Propyl, Biphenyl, Ultraaqueous C18

Trident HPLC Column Protection System *New Methods Development & Validation KitsHPLC Applications (PDF files*

* see PDF file for more complete details

More LC-MS HPLC Columns:

• Ultra C8

• Ultra C4

• Ultra C1

• Ultra PFP

• Ultra Phenyl

• Ultra Cyano

• Ultra Amino

• Ultra Silica

• Allure Silica

Ultra
The Ultra family of HPLC packings is bonded and end-capped at maximum density on 3m and 5m, 100, pure, Type B silica particles.The high-percentage carbon and phase coverage provides heightenedsolute retention, which makes these packings ideal for liquidchromatography/mass spectrometry (LC/MS) applications. Additionally,the bonding chemistry of each Ultra phase is very reproducible andreliable

Allure
Unique bonding chemistry and 60 Type B silica particles set the Allure family of HPLC packings in acategory by themselves for LC/MS column technology. Research and development of these phases have concentrated on obtaining maximum retention for analytes based on their functional group moieties, without compromising quality and reproducibility. The research evolved into the formulation of three superior products - Allure C18, Allure Basix, and Allure Acidix columns - that target four classes of analytes as diagrammed above. Allure C18 columns contain maximum bonded phase coverage with 27% carbon load, and superior retention of neutral to slightly polar mixtures including steroids, pesticides, and synthetic intermediates. It also has been found to separate all 14 EPA Method 8330 explosives (the method calls for two columns to accomplish this) in less than 10 minutes.
The Allure Basix column is designed to separate compounds containing amine(s) moiety, with maximum retention. This characteristic is ideal for analyzing many pharmaceutical products.

You have the application, we have the LC/MS column.

The Allure Acidix columns provide maximum retention and selectivity of analytes with carboxylic, phosphoric, sulfonic, and other acid group functionalities. This column also stands alone in providing excellent peak shape and retention for amino acids, without the need for derivatization. Furthermore, the Allure Acidix column retains the more difficult, highly polar solutes, where C18 phases fail to perform. The Ultra IBD phase contains unique bonding chemistry and performs well with all target classes. This intrinsically basedeactivated (IBD) phase contains a polar group within a hydrocarbon chain, which participates in the retention and separation process. It also assists in deactivating the silica surface to reduce peak tailing of strong basic analytes.
The Allure Basix, Allure Acidix, and Ultra IBD packings can be used in both normal phase (NP) and reverse phase (RP) mode (Figure 2). Even with traditional reverse phase solvents (e.g., water:acetonitrile), these packings behave as both NP and RP, depending on the solvent ratio. This offers a tremendous advantage in LC/MS separations where high organic/less aqueous mobile phase content is desirable. More organic can be added to the mobile phase, yet the compounds will actually increase in retention. This retains the solutes away from the solvent front to prevent solvent interference, while simultaneously increasing the MS sensitivity. We have already seen increased LC/MS sensitivities of more than 250% using this concept!

Figure 2: Cephaloridine Retention Time vs. % Acetonitrile at pH 3.0 (20mM Ammonium Formate)

Applications (ALL)
Allureâ„¢ Acidix
LC/MS Analysis of Ascorbic Acid on Allure Acidix
Amino Acids on Allure Acidix
Adenine Nucleotides on the Allure Acidix
Amino Acids on Allure Acidix
Acetaminophen on Allure Acidix
Aspirin and Salicylic Acid on Allure Acidix
Ibuprofen and Naproxen on Allure Acidix
Organic Acids on Allure Acidix
Allureâ„¢ Basix
LC-MS Analysis of Tricyclic Antidepressants on Allure Basix
Ethinyl Estradiol and Norgestrel on Allure Basix
Ethynyl Estradiol and Norethindrone on Allure Basix
Ibuprofen and Pseudoephedrine on Allure Basix
Caffeine, Acetaminophen, and Pseudoephedrine on Allure Basix
Timolol Maleate on Allure Basix

Pinnacle Specialty Phases

• Pinnacle TO-11 for Carbonyls

• Pinnacle EcoSep exhibits excellent selectivity for uron herbicides.

• Pinnacle Carbamate column offers optimized selectivity for free carbamate pesticides.

General Purpose Columns . . .
NEW 2001 PINNACLE11 Pinnacle11 C18, Phenyl, Cyano, C8 - higher performanceReduced cost ( approx Aud$350.00 )Controlled uniform pore sizeHigher EfficiencyMore inert - due to ultra high purity silica base materialIn-house product of ALL materials QCExcellent batch-to-batch reproducibility

Ultra IBD
Carbamtes on Ultra IBD
Uracil, Uridine, UTP, UDP, and UMP on Ultra IBD
Thymine, Thymidine, TTP, TDP, and TMP on Ultra IBD
Guanine, Guanosine, GTP, GDP, and GMP on Ultra IBD
Cytosine, Cytidine, CTP, CDP, and CMP on Ultra IBD
Adenine, Adenosine, ATP, ADP, and AMP on Ultra IBD
11 Component  Mixture of Nucleotides, Nucleosides, and Nitrogenous Bases on Ultra IBD
LC/MS of Penicillin V on Ultra IBD
LC/MS of Cephradine on Ultra IBD
LC/MS of Cephaloridine on Ultra IBD
LC/MS Analysis of Cimetidine on Ultra IBD
Cephaloridine by Reverse Phase on Ultra IBD
Cephaloridine Normal Phase Separation on Ultra IBD
Penicillin V on Ultra IBD
Cephalexin and Cephradine on Ultra IBD
Amoxicillin on Ultra IBD
Digoxin on Ultra IBD
Diflubenzuron on Ultra IBD
Furosemide on Ultra IBD
Pyridine and Phenol on Ultra IBD at pH 7.0
Acids and Bases at pH 3.0 on Ultra IBD
Acids and Bases at pH 7.0 on Ultra IBD
Ultra PFP
Levothyroxine on Ultra PFP
Ultra Phenyl
Guaifenesin with Codeine on Ultra Phenyl
Ultra Cyano
EPA 8330 Explosives on Ultra Cyano
Beclomethasone on Ultra Cyano
Calcium Channel Blockers on Ultra Cyano
Ultra C4
Hormones on Ultra C-4
Ultra Aqueous C18
Organic Acids on Ultra Aqueous C18
Ultra C18
EPA Method 8316 on Ultra C18
Carbonyls: DNPH derivatives on Ultra C18
Ÿ-Estradiol, Norethinedrone & Ethynyl Estradiol on Ultra C18
Beclomethasone & des-chlorobeclomethasone on Ultra C18
Ÿ-Estradiol, Norethinedrone & Ethynyl Estradiol on Ultra C18
Corticoids on Ultra C18
EPA Method 8316 on Ultra C18
Carbonyls: DNPH derivatives on Ultra C18
Carbonyls on Ultra C18

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Analyte Functional Group:

Restek HPLC Columns :

Acid

Allure Acidix | Ultra IBD

Base

Allure Basix | Allure PFP Propyl | Ultra IBD

Neutral:

Hydrophobic

Ultra C18 | Allure C18

Hydrophilic

Ultra Aqueous C18 | Ultra IBD

Mixed
(acids, bases, & neutrals)

Ultra IBD | Allure Basix

HPLC Applications - Chromatograms

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