High-Throughput Extractions of Small Molecules Directly from Biological Fluids:

 No protein crashing, emulsion formation, dry wells or solvent exchange

• Easily automated
• Does not require a vacuum or pressure system
• Minimal user management, especially on a well-to-well basis
• Minimized solvent usage and disposal
• High precision, efficiency and capacity

Immobilized Liquid Extraction™ (ILE) Well Plates . . . 

          are the perfect solution to your high-throughput extraction needs.
ILE Well Plates provide analysts reliable, rapid and environmentally friendly high-throughput extractions without protein crashing, emulsion formation, solvent exchange or dry wells.
ILE Well Plates involve minimal user management, especially on a well-to-well basis, do not require vacuum or pressure systems, and are capable of extracting small molecules directly from blood, serum or any aqueous biological matrix.

• Each well of an ILE Well Plate is coated with a thin layer of polymer (immobilized liquid) which acts as the medium of extraction. When a sample is directly exposed to the polymer, targeted analytes partition between the aqueous sample and the immobilized phase at a predictable rate.

• This partitioning process is allowed to occur until extraction equilibrium is attained.

• The quantity of a specific analyte that is extracted by the immobilized phase is determined both by the analyte's relative affinity for both the sample and the immobilized phase (partition ratio), as well as by the relative volumes of the sample and the extracting polymer (phase ratio).

• The partitioning process may be accelerated by agitating the ILE Well Plate with a vortexer, sonicator or another appropriate method. Proper agitation disrupts a boundary layer which forms at the sample-polymer interface, a consequence which may alternatively be accomplished by repeatedly dispensing and aspirating a sample into and out of each well.

• Analytes which partition into the polymer are 'back-extracted', or desorped, into a small amount of LC or GC solvent,
  at which point the extraction is complete.

New ILE Well Plate Applications! (Ask !)

Example of Simple 3-Step Well Plate Procedure

(1) Extract: Load samples into appropriate wells and thoroughly agitate (vortex, sonicate, etc.)
ILE well-plate. Proper agitation ensures the disruption of a boundary layer which forms at the sample-polymer interface.
This process is continued until reaction equilibrium is reached, or nearly reached and analytes have sufficiently partitioned
from the sample into the immobilized phase.

(2) Back-Extract: Dispense a small amount of method-appropriate solvent into each well -- a sufficient quantity of solvent will cover the entire coated portion of each ILE well. Compounds partition between the immobilized polymer and the solvent in accord with their relative affinities for each. This partitioning may be accelerated by agitating the well, and is allowed to occur until equilibrium is reached, or nearly reached.

(3) Analyze: The remaining solvent in each well may be immediately analyzed by GC or HPLC, or the ILE Well Plate
may be sealed and archived for later analysis.

ILE 96-Well Plate Cat.#s / Prices

96-Well Extraction Kits are a risk-free way to develop / implement a method for your specific application.
Based on Immobilised Liquid EXtraction the 96-well plate technology is immediately and readily scaled up for
a semi-automation / batch extraction from ILE Cap technology.
ILE Caps are recommended as a protyping step in your method development

Accessories
include Orbital Shaker for 96-Well Plates ; ILE Plate Orbital Shaker  PDF ~Aud795.00)
PAL Autosampler Systems for GC ( not currently available from Chromalytic Tech )

ILE 96-Well Method Development Kit - Immobilized Liquid Extraction

		PRICE/QTY @ 1xUSD=0.8xAUD
Product ID	Description	1	5	50
WU96-MDK3	ILE 96-Well Method Development Kit	Aud450.00	N/A	N/A

For $450, each kit includes three ILE Well Plates (one of each phase), and full method development assistance.

If you are unable to develop an appropriate method, simply return the plates to us within thirty days of receipt for a full refund. Or, if you decide to place an order for additional plates, the original purchase price of $450 will be credited toward your order.

More information about custom method development. (Ask !)

		1	5	50
WU96-M003	ILE 96-Well Plate; PDMS, 0.3uL.	Aud310.00	*Enquire !	*Enquire !

Polydimethylsiloxane (PDMS) - Relatively non-polar phase for extracting hydrophobic compounds. It has been experimentally shown that PDMS exhibits polarity and extractive properties similar to n-octanol. It is recommended as a general purpose ILE phase and is suitable for a wide range of extractions.

WU96-P003

ILE 96-Well Plate; Phenyl, 0.3uL.

$310.00

*Enquire !

*Enquire !

Polydiphenylsiloxane (Phenyl) - This is a more polar phase, and would be expected to display enhanced selectivity for aromatic compounds due to pi-bonding potential - a similar effect to extracting with toluene.

WU96-F003

ILE 96-Well Plate; Fluoro, 0.3uL.

$310.00

*Enquire !

*Enquire !

Trifluoropropylmethylsiloxane (Fluoro) - This phase provides enhanced selectivity for extracting compounds with lone pairs of electrons.

Prices are approx AUD ( @ USD=0.8Aud )
*Attractive Discount for Quantity Orders