Hydrophobic Interaction Columns

HYDROCELL HPLC Column

Hydrophobic Interaction Chromatography (HIC) separates proteins by mechanisms  similar to Reversed-Phase Chromatography but under gentle reverse salt gradient  elution conditions in aqueous buffers. Since no organic solvent is used,  biological activity has a much higher probability of being retained.

HYDROCELL C3 1500, C4 1500 and phenyl 1500 are prepared from 10 µm particles  of hydrophilic PS-DVB beads with pore diameter of 500 Ã…. Similiarly, C3 3000, C4  3000 and Phenyl 3000 are produced from 10 µm particles of hydrophilic,  macroporous PS-DVB beads with average pore diameter of 1500Ã….
The packing  materials have been chemically bonded with allyl (C3), butyl (C4) or phenyl as a  hydrophobic group. These packings are the same as HYDROCELL ion exchange  packings which do not have any weak ester linkages as do the other commercial  products.
They are stable in a pH range of 1 to 13 and are compatible with most  organic solvents.
The columns can be operated at high flow rates for rapid  equilibration and fast clean-up.

The advantages are:

• Retain Biological Activity
• High Resolution and Efficiency
• Excellent Protein Recoveries

HYDROCELL HIC Columns are available in both stainless steel and PEEK.
Please  refer to Ordering Info for available column size and  catalog order number information.

The dynamic protein loading capacity determined by Frontal Uptake procedures  are about 10 mg, 13 mg and 15 mg of lysozyme per mL of column volume on C3 1500,  C4 1500 and Phenyl 1500 respectively. The practical loading capacity is about  0.2 - 0.3 mg of protein on a 50 x 4.6mm column. Detailed chromatograms are available see DJVu Catalog

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