Commonly Asked Questions - What products are included in the kits?
Each methods development kit package contains four columns (Allure™ C18, Allure™ Acidix, Allure™ Basix, and Ultra IBD) to provide a wide range of selectivity. The kit for HPLC methods development contains the most popular size for HPLC analysis (150x4.6mm). The LC/MS kit contains the columns most frequently used for LC/MS applications (50x2.1mm).
Methods validation kits contain three columns, each packed from different lots of the stationary phase. The validation
kits are available for each of Restek's four most popular phases found in the methods development kit package and in two dimensions -- 150x4.6mm for HPLC methods and 50x2.1mm for LC/MS methods.
- How does Restek ensure reproducibility?
To ensure that Restek's phases are reproducible, a minimum of three lots of a stationary phase are verified
for consistency before any new HPLC column is commercially released. Each lot of material must meet stringent chromatographic reproducibility requirements before release, and each column is individually tested before shipment.
- How do the different phases in the kits affect selectivity?
The Allure™ C18 phase, Ultra IBD phase, Allure™ Basix phase, and the Allure™ Acidix phase provide a wide range of selectivity based upon the pKa or polarity of the analyte.
The Allure™ C18 phase is ideal for retention of neutral, strongly lipophilic analytes. The high 27% carbon load of the Allure™ C18 phase results in maximum retention of lipophylic analytes.
The IBD
phase has many of the separation characteristics of a C18 phase and will behave much like a C18 phase in reverse phase mode, although selectivity often will be enhanced. The IBD phase's special functionality also can create additional retention for analytes containing polar groups, cations, or anions, which can be poorly retained by C18 phases.
For even greater retention of acids and bases, the
Allure™ Acidix and Allure™ Basix phases, respectively, are the columns of choice.
The Allure™ Acidix phase is engineered to provide maximum retention of acids and anions. The Allure™ Acidix phase retains amino acids without derivitization and will retain other short to medium chain length organic acids.
The Allure™ Basix
phase provides maximum retention of bases and cations. These stationary phases display their own unique retention profiles and do not mimic the Allure™ C18 phase at any point in the separation. It is important to remember that neither the Allure™ Acidix nor the Allure™ Basix phase are ion exchange packings. They retain analytes by polar interaction.
- Can I order a kit customized to fulfill my company's specific analytical needs?
Custom kits containing columns in the dimensions of your choice are available upon request. Kits that contain other phases are also available. For more information on custom methods development or validation kits, contact us
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