• LC-MS - short, small ID

• general purpose screening - use 150mm

• Larger ID for increased sample capacity

• small ID use may be limited by your HPLC hardware and detector dead volume

HPLC Applications - Chromatograms
( see DJVu catalog )

Amino Acids Nucleic Acid Bases, Nucleosides Nucleotides 

Column Phase

Cat Page

adenine

Ultra IBD

43

adenosine

Ultra IBD

43

ADP

Ultra IBD

43

AMP

Ultra IBD

43

ATP

Ultra IBD

43

CDP

Ultra IBD

43

CMP

Ultra IBD

43

cytidine

Ultra IBD

43

guanine

Ultra IBD

43

guanosine

Ultra IBD

43

homocysteine

Allure Acidx-LC/MS

43

homocystine

Allure Acidx-LC/MS

43

homocysteine thiolactone

Allure Acidx-LC/MS

43

methionine

Allure Acidx

43

phenylalanine

Allure Acidx

43

TMP

Ultra IBD

43

tyrosine

Allure Acidx

43

adenine

Allure Acidx

44

adenosine

Ultra IBD

44

ADP

Allure Acidx

44

AMP

Allure Acidx

44

ATP

Allure Acidx

44

CMP

Ultra IBD

44

CTP

Ultra IBD

44

cytidine

Ultra IBD

44

cytosine

Allure PFP Propyl

44

adenine

Allure PFP Propyl

45

cytosine

Allure PFP Propyl

45

GDP

Ultra IBD

45

GMP

Ultra IBD

45

GTP

Ultra IBD

45

guanine

Allure PFP Propyl

45

guanosine

Ultra IBD

45

TDP

Ultra IBD

45

thymidine

Ultra IBD

45

thymine

Allure PFP Propyl

45

TMP

Ultra IBD

45

TTP

Ultra IBD

45

UDP

Ultra IBD

45

UMP

Ultra IBD

45

uracil

Allure PFP Propyl

45

UTP

Ultra IBD

45

Explosives

4-amino 26-dinitrotoluene

Ultra Cyano/Allure C18

46

2-amino 46-DNT

Ultra Cyano/Allure C18

46

13-dinitrobenzene

Ultra Cyano/Allure C18

46

24-DNT

Ultra Cyano/Allure C18

46

26-DNT

Ultra Cyano/Allure C18

46

13-DNB

Ultra Cyano/Allure C18

46

24-DNT

Ultra Cyano/Allure C18

46

26-DNT

Ultra Cyano/Allure C18

46

34-DNT (Int. std.)

Ultra Cyano/Allure C18

46

HMX

Ultra Cyano/Allure C18

46

nitrobenzene

Ultra Cyano/Allure C18

46

2-nitrotoluene

Ultra Cyano/Allure C18

46

3-NT

Ultra Cyano/Allure C18

46

4-NT

Ultra Cyano/Allure C18

46

2-NT

Ultra Cyano/Allure C18

46

4-NT

Ultra Cyano/Allure C18

46

3-NT

Ultra Cyano/Allure C18

46

RDX

Ultra Cyano/Allure C18

46

tetryl

Ultra Cyano/Allure C18

46

135-TNB

Ultra Cyano/Allure C18

46

246-trinitrotoluene

Ultra Cyano/Allure C18

46

135-TNT

Ultra Cyano/Allure C18

46

246-TNT

Ultra Cyano/Allure C18

46

PAH/Carbonyls-DNPH Derivs

acetaldehyde

Ultra C18

47

acetone

Ultra C18

47

acrolein

Ultra C18

47

benzaldehyde

Ultra C18

47

butyraldehyde

Ultra C18

47

crotonaldehyde

Ultra C18

47

formaldehyde

Ultra C18

47

hexanaldehyde

Ultra C18

47

Methyl ethyl ketone

Ultra C18

47

methacrolein

Ultra C18

47

m-tolualdehyde

Ultra C18

47

propionaldehyde

Ultra C18

47

valeraldehyde

Ultra C18

47

Pesticides/Herbicides

aldicarb

Ultra IBD

47

aldicarb sulfoxide

Ultra IBD

47

aldicarb sulfone

Ultra IBD

47

carbaryl

Ultra IBD

47

carbofuran

Ultra IBD

47

diflubenzuron

Ultra IBD

47

3-hydroxycarbofuran

Ultra IBD

47

methiocarb

Ultra IBD

47

NN' bis-(chlorobenzyl) urea

Ultra IBD

47

oxamyl

Ultra IBD

47

propoxur

Ultra IBD

47

methomyl

Ultra IBD

47

PAH/Carbonyls-DNPH Derivs

Column Phase

Cat Page

acetaldehyde

Ultra C18

48

acetone

Ultra C18

48

acrolein

Ultra C18

48

benzaldehyde

Ultra C18

48

butyraldehyde

Ultra C18

48

crotonaldehyde

Ultra C18

48

formaldehyde

Ultra C18

48

hexanaldehyde

Ultra C18

48

Methyl ethyl ketone

Ultra C18

48

methacrolein

Ultra C18

48

m-tolualdehyde

Ultra C18

48

propionaldehyde

Ultra C18

48

valeraldehyde

Ultra C18

48

Drugs of Abuse 

COC (Cocaine)

Allure PFP Propyl

48

EME (ecgonine methyl ester)

Allure PFP Propyl

48

amitdptyline

Ultra C18

48

Free Acids

acetic acid

Ultra Aqueous C18

48

benzoic acid

Ultra Aqueous C18

48

citric acid

Ultra Aqueous C18

48

fumaric acid

Ultra Aqueous C18

48

nortriptyline

Ultra Aqueous C18

48

lactic acid

Ultra Aqueous C18

48

malonic acid

Ultra Aqueous C18

48

succinic acid

Ultra Aqueous C18

48

ascorbic acid

Allure Acidx LC/MS

49

Antidepressants  

trimipramine

Ultra C18

48

amitdptyline

Ultra IBD @ ph7

49

nortriptyline

Ultra IBD @ ph7

49

trimipramine

Ultra IBD @ ph7

49

Organic Bases

phenol

Ultra IBD @ ph7

49

pyridine

Ultra IBD @ ph7

49

Analgesics

acetaminophen

Allure Acidix

50

aspirin

Allure Acidix

50

caffeine

Allure Basix

50

codeine

Allure Basix

50

hydroxycodone

Allure Basix

50

oxycodone

Allure Basix

50

salicylic acid

Allure Acidx

50

aspirin

Allure Acidix LC/MS

51

ibuprofen

Allure Basix

51

naproxen

Allure Acidx

51

salicylic acid

Allure Acidix LC/MS

51

codeine

Ultra Phenyl

56

Antihistamines

pseudoephedrine

Allure Basix

50

pseudoephedrine

Allure Basix

51

cimetidine

Ultra IBD

55

Antibiotics

amoxicillin

Ultra IBD

51

cephalexin

Ultra IBD

52

cephaloridine

Ultra IBD

52

cephradine

Ultra IBD

52

cephradine

Ultra IBD LC/MS

53

penicillin V

Ultra IBD

53

sulfamethoxazole

Allure Basix

53

sulfamethoxazole

Allure Basix

54

trimethoprim

Allure C18

sulfamethoxazole

Sedatives

alprazolam

Allure C18

Iorazepam

Allure Basix

54

cyclobenzaprine HCI

Allure Basix

62

glyburide

Ultra IBD

55

Antidepressants  

desipramine

Allure Basix LC/MS

54

sertraline HCI

Allure Basix

54

trimipramine

Allure Basix LC/MS

54

amitdptyline

Allure Basix

55

desipramine

Allure Basix

55

doxepin

Allure Basix

55

imipramine

Allure Basix

55

nordoxepin

Allure Basix

55

nortriptyline

Allure Basix

55

protriptyline

Allure Basix

55

Antitussive  

guaifenesin

Ultra Phenyl

56

Asthma 

albuterol

Allure Basix

56

Cardiac  

atenolol

Allure Basix

56

diltiazem

Ultra Cyano

56

nicardipine

Ultra Cyano

56

nifedipine

Ultra Cyano

56

verapamil

Ultra Cyano

56

digoxin

Ultra IBD

57

enalapril

Allure Basix

57

furosemide

Allure Basix

57

maleate

Allure Basix

57

hydrochlorothiazide

Allure Basix

58

maleate

Allure Basix

58

metoprolol

Allure Basix

58

timolol

Allure Basix

58

triamterene

Allure Basix

58

verapamil HCI

Allure Basix

59

Steroids and Hormones

b-estradiol

Ultra C18

59

ethynyl estradiol

Ultra C18

59

norethindrone

Ultra C18

59

ethynyl estradiol

Allure Basix

60

medroxyprogesterone acetate

Allure Basix

60

medroxyprogesterone

Ultra C4

60

norethindrone

Allure Basix

60

norgestrel

Allure Basix

60

b-estradiol

Ultra C4

61

ethynyl estradiol

Ultra C4

61

ethynyl estradiol

Allure C18

61

norethindrone

Ultra C4

61

norgestrel

Allure C18

61

norgestrel

Ultra C18

61

beclomethasone

Ultra C18

62

deschlorobeclomethasone

Ultra C18

62

beclomethasone

Ultra Cyano

63

corticosterone

Ultra C18

63

deoxycorticosterone

Ultra C18

63

deschlorobeclomethasone

Ultra Cyano

63

dexamethasone

Ultra C18

63

hydrocortisone

Ultra C18

63

triamcinolone

Ultra C18

63

corticosteroids

Allure C18

64

  MiniCat Subsection Home

HPLC Column Catalog 2001 # 59241

DJVCu Catalog 68p
Applications
HPLC Applications : 42-64
HPLC FREE Literature : 66
also see Restek PINNACLE HPLC Columns and the new lower cost general purpose Pinnacle 11 columns

HPLC Application / Chromatograms

Allure 60A pore surface area
Ultra 100A pore size
C18 - most commonly used phase for neutral molecules
Compatible with polar organic and aqueous mobile phases - large library of applications

Ultra C18 - for neutral hydrophobic analytes
Allure C18 - designed for LC-MS
high surface area / high ligand density (27% C) leads to use of higher concentration of organic solvent and  enhanced electrospray MS sensitivity
Ultra Aqueous C18 - enhanced  retention and selectivity for polar analytes ( neutral to mildly acidic)
Ultra IBD ( intrinsically base deactivated) - enhanced retention of hydrophyllic and highly acidic analytes
The best phase for simultaneously  analysing neutral, acid, basic compounds

Allure Acidx - for highly acidic analytes usually in normal phase mode with predominantly methanol/acetonitrile for enhanced LC-MS sensitivity

Allure Basix - for basic compounds, amine containing pharmaceuticals, reverse phase LC-MS, and for simultaneously  analysing neutral, acid, basic compounds

HPLC Columns and Accessories

Table of Contents
HPLC Column Selection :4-10
US Pharmacopoeia Cross Reference : 11

ALL common sizes
L 50, 100, 150, 250, 300mm
ID 4.6mm, 3.2mm, 2.1mm 1.0mm
3 and 5micron particles

Allure Acidex Columns : 12
Allure Basix Columns (USP L10) : 13
Allure C18 (USP L1) : 14
Ultra C18 Columns (USP L1) : 15
Ultra Aqueous C18 Columns (USP L1) : 16
Ultra IBD Columns Columns : 17
Allure PFP Columns : 18
Ultra PFP Columns : 19
Ultra C8 Columns (USP L7) ; 20
Ultra C4 Columns (USP L26) : 21
Ultra C1 Columns (USP L13) : 22
Ultra Phenyl Columns (USP L11) : 23
Ultra Cyano (CN) (USP L10) : 24
Ultra Amino (NH2) : 25
Allure Silica Columns (USP L3) : 26
Ultra Silica Columns (USP L3) : 27

Methods Development Kits : 28
Kromasil Columns : 28
Guard Columns : 29-31
HPLC Accessories & Organisers : 32-41

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