Chromatography FAQs

 GC and HPLC *.PDF

Restek ?

 - What Sets Restek Columns Apart from the Competition

NEW from
Walt Jennings

• The Replacement Column, A Recurring Problem in GC : ADV 2006 v1

• Preventive Maintenance for GC : ADV 2006 v2

GC and Capillary GC


 

• How to Become Proficient in Troubleshooting GC Systems

• How Do YOU Select the Best Column for YOUR Analysis

• What are the Advantages of Different Column Materials

• What Type of Inlet Liner is Best for YOUR Analysis

• Vaporising Split Injectors

• What is Septum Bleed and How do YOU Minimise It
  -  When is Column Bleed Normal and When is it a Problem

• Column Overload in Capillary GC ?

• What are MS Grade Capillary Columns Rtx-5MS

• . . . NEW MS Columns 99

• Using Flame Ionisation Detectors

• Using ECD Detectors

• Using ELCD Detectors   
  Innovative Sample Extraction products                      

Koni's Korner *

• The Value of Education

• Certification of Injectors and Injection Techniques
       - Comments on splitless injection by readers

• Sample Evaporisation in Hot Injectors - A Problem ?

• Why Uncoated Capillary Columns allow Large Injections

• Are GC Techniques Really Optimised - Splitless Injection                                                         

Copyright 1992-1999 - Articles by Dr Konrad Grob and Prof Walt Jennings- All rights reserved repectively

HPLC Faqs

NEW Silica materials ADVANTAGES

• Use High Purity Silica - low metallic contect; improved analysis of "difficult" polar samples

• Highly uniform particle size - better column efficiency

• A trend to smaller ID and smaller particles size; reduced length / presure drop and solvent consumption

• Use of micropolished tubing and / or glass-lined

What's Wrong with C18 phases ?

• Whilst being the standard HPLC phase :

• Results tend to be of poor reproducibiliy particularly using highly aqueous solvents and analysis of of polar samples

• Explanation - due to what has been reported as "chain fold-back/collapse" but also the phenomena is due to a phase transition for C18-Si-OH between 15-30degC
  Answers use . . .

• C18 using an "embedded polar phase" silica linkage -  Restek, GROM Analytik

• C16+C2 - a branched C18 ChromAB Corp Phase transition reduced to 0-10 degC

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