Selecting a Pre-Packed Column1. Choose the particle size and pore size HYDROCELL
media are available in 5 µm, 10 µm, 20 µm, 30 µm and 50 µm particle size of macroporous media for routine ion exchange, hydrophobic interaction, reversed phase and nucleic acid analysis and purification.
Hydrocell media are also produced nonporous polymeric beads for high-speed analysis and process control |
Particle Size | Pore Size | Applications | 5 µm | 500Å, 1500Å | High resolution, Reversed Phase media for protein, polypeptide and oligonucleotide analysis, and small-scale separation. | 10 µm | 500Å, 1500Å | Ion Exchange, Hydrophobic interaction and Reversed Phase media for analytical to small-scale preparative | 10 µm | nonporous | Same as 10 µm porous particle except producing from nonporous beads for high speed analysis and preparation. | 20 µm | 500Å, 1500 Å | Same as 10 µm porous particle except for high pressure semi-preparative to preparative applications |
30 µm | 500Å, 1500 Å | Same as 10 µm porous particle except for medium and low pressure preparative and process applications | 50 µm |
500Å, 1500 Å | Same as 10 µm particles except for medium and low pressure preparative and process applications |
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2. Select the column geometry HYDROCELL
columns are available in several sizes. We recommend 4.6 mm ID column for routine lab scale use.
For semi-preparative and preparative work choose a larger diameter 7.8 mm, 10 mm or 21.2 mm column.
For small scale work or more sensitive detection, choose a narrow-bore 1.0 or 2.1 mm ID column.
All standard Hydrocell 1500, 3000 and NP10 series of columns are constructed of PEEK or Stainless steel materials with 2 µm Titanium frit |
Scale of Separation | Column Dimension | Recommended Flow | | Diameter (mm) | Length (cm) | Volume (mL) | Volumetric (mL/min) | Line velocity (cm/hr) | Micro (µg) | 2.1 | 15 | 0.52 | 0.20-1.20 | 350-2100 | Lab (µg to mg) | 4.6 | 15 | 2.50 | 1.00-6.00 | 360-2200 | | 7.8 | 15 | 7.16 | 2.50-15.0 |
310-1860 | Semi-Preparative (mg) | 10 | 15 | 11.50 | 4.00-24.0 | 310-1860 | Preparative (mg to grams) | 21.2 | 15 | 50.60 | 15.0 -90 | 255-1530 | |
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3. Match the Column to the System Each pre-packed HYDROCELL 1500, 3000 or NP10 series of columns are produced from 10 µm and 20 µm particle size with average pore size of 500Å, 1500 Å or nonporous PS-DVB polymeric beads for high pressure systems.
HYDROCELL 30 and
50 series of columns are designed for medium and low pressure preparative systems.
Choose the column that is suitable for your system environment. This will ensure that the back pressure generated on the column will not exceed the pressure limit of your system while operating under higher flow rates. Fig. 1 - Pressure/Flow Relationship of NP10 series, 150x4.6mm columns
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Fig. 2 - Pressure/Flow Relationship of 1500 series, 150x4.6mm columns
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Fig. 3 - Pressure/Flow Relationship of Reversed Phase RP 10G, 150X4.6 mm column |
4. Choose the HYDROCELL Chemistry |
Product | Functional Group | Particle Size (µm) |
Pore Size (A) | Dynamic
Loading Capacity
(column volume/mL) |
Ion Exchange Chromatography |
DEAE 1500 | Diethylaminoethyl | 10 | 500 | 50 mg | DEAE 3000 |
Diethylaminoethyl | 10 | 1500 | 45 mg | DEAENP10 | Diethylaminoethyl | 10 | Non-porous | 35mg | QA 1500 | Diethylmethylaminoethyl | 10 | 500 | 50 mg | QA 3000 | Diethylmethylaminoethyl | 10 | 1500 | 45 mg | QA NP10 | Diethylmethylaminoethyl | 10 | non-porous | 35 mg | CM 1500 | Carboxymethyl | 10 | 500 | 45 mg | CM 3000 | Carboxymethyl | 10 | 1500 | 40 mg | CM NP10 |
Carboxymethyl | 10 | non-porous | 25 mg | SP 1500 | Sulfopropyl | 10 | 500 | 45 mg | SP 3000 | Sulfopropyl | 10 | 1500 | 40 mg | SP NP10 | Sulfopropyl | 10 |
non-porous | 25 mg | NS 1500 | Quaternized Amine | 10 | 500 | 60 mg
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Hydrophobic Interaction Chromatography | C3 1500 | Allyl | 10 | 500 | 30 mg | C3 3000 | Allyl | 10 | 1500 |
25 mg | C3 NP10 | Allyl | 10 | non-porous | 15 mg | C4 1500 | Butyl | 10 | 500 | 30 mg | C4 3000 | Butyl | 10 | 1500 | 25 mg | C4 NP10 | Butyl | 10 | non-porous | 15 mg | Phenyl 1500 | Phenyl | 10 | 500 | 35 mg | Phenyl 3000 | Phenyl | 10 | 1500 | 30 mg | Phenyl NP10 | Phenyl | 10 | non-porous | 20 mg |
Reversed-Phase Chromatography | RP 5T | Base PS-DVB | 5 |
140 | 60 mg | RP 5G | PS-DVB with coating | 5 | 500 | 45 mg |
RP 10G | PS-DVB with coating | 10 | 500 | 40 mg | RP 5S | Base PS-DVB | 5 | 500 | 50 mg | RP 10S |
Base PS-DVB | 10 | 500 | 45 mg | RP 5D |
Base PS-DVB | 5 | 1500 | 45 mg | RP 10D | Base PS-DVB | 10 | 1500 | 40 mg |
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