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HPLC Column Selection

The Allure Acidix columns provide maximum retention and selectivity of analytes with carboxylic. phosphoric. sulfonic. and other acid group functionalities. This column also stands alone in providing excellent peak shape and retention for amino acids, without the need for derivatization. Furthermore, the AllureTM Acidix column retains the more difficult, highly polar solutes, where C 18 phases fail to perform.

The Ultra IBD phase contains unique bonding chemistry and performs
well with all target classes.
This intrinsically base-deactivated (IBD) phase contains a polar group within a hydrocarbon chain, which participates in the retention and separation process. It also assists in deactivating the silica surface to reduce peak tailing of strong basic analytes.

The AIlure Basix, Allure Acidix, and
Ultra IBD packings can be used in both normal phase (NP) and reverse phase (RP) mode (Figure 2).

Even with traditional reverse phase solvents (e.g., water:acetonitrile),
these packings behave as both NP and RP. depending on the solvent ratio.
This offers a tremendous advantage in LC/MS separations where high organic/less aqueous mobile phase content is desirable.
More organic can be added to the mobile phase. yet the compounds will actually increase in retention.
This retains the solutes away from the solvent front to prevent solvent interference, while simultaneously increasing the MS sensitivity.
We have already seen increased LC/MS sensitivities of more than 250% using this concept!